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Figure 1. Characterization of the purified serotype A and O FMDV. The purity and particle structures were identified by (A) SDS-PAGE, (B) <t>TEM,</t> and (C) <t>HPSEC.</t> (D) The DSF signals and schematic illustration of the RNA release and binding with the florescent dye to trigger a strong fluorescence signal upon the thermal-induced dissociation of 146S. (E) The first-derivative plots of the fluorescence curves of the FMDV. The FMDV after DSF scanning up to 65 ◦C was reanalyzed by (C) HPSEC.
Hpsec Analyses Tem, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hpsec analyses tem - by Bioz Stars, 2026-08
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Figure 1. Characterization of the purified serotype A and O FMDV. The purity and particle structures were identified by (A) SDS-PAGE, (B) TEM, and (C) HPSEC. (D) The DSF signals and schematic illustration of the RNA release and binding with the florescent dye to trigger a strong fluorescence signal upon the thermal-induced dissociation of 146S. (E) The first-derivative plots of the fluorescence curves of the FMDV. The FMDV after DSF scanning up to 65 ◦C was reanalyzed by (C) HPSEC.

Journal: Vaccines

Article Title: Simple and High-Throughput Quantification of Mono- and Bivalent Foot-and-Mouth Disease Virus Vaccine Antigens by Differential Scanning Fluorimetry

doi: 10.3390/vaccines13070721

Figure Lengend Snippet: Figure 1. Characterization of the purified serotype A and O FMDV. The purity and particle structures were identified by (A) SDS-PAGE, (B) TEM, and (C) HPSEC. (D) The DSF signals and schematic illustration of the RNA release and binding with the florescent dye to trigger a strong fluorescence signal upon the thermal-induced dissociation of 146S. (E) The first-derivative plots of the fluorescence curves of the FMDV. The FMDV after DSF scanning up to 65 ◦C was reanalyzed by (C) HPSEC.

Article Snippet: TEM and HPSEC Analyses TEM used a Philips FEI Tecnai 20 TEM (Royal Philips Electronics, Amsterdam) after the samples were applied to a 400-mesh copper grid and negatively stained with 1% (v/v) uranyl acetate.

Techniques: Purification, SDS Page, Binding Assay, Fluorescence